• Sonuç bulunamadı

PLANT KARYOLOGICAL TECHNIQUES

N/A
N/A
Info
İndir
Protected

Academic year: 2021

Share "PLANT KARYOLOGICAL TECHNIQUES"

Copied!
13
0
0

Yükleniyor.... (view fulltext now)

Şimdi indir ( 13 sayfa )

Tam metin

(1)
(2)

karyotype

• The number and appearance of chromosomes in the nucleus of a eukaryotic cell.

• complete set of chromosomes in a species or in an individual organism and for a test that detects this complement or

(3)

Pretreatment

• well-spread, • clear,

(4)

Pretreatment

• Increases the number of metaphase cells • Constricts the chromosomes longitudinally • Clarifies the constrictions

(5)

Microtubule-inhibiting pretreatment

substances

• colchicine,

• 8-hydroxyquinoline, • p-dichlorobenzene, • ice cold water,

(6)

FIXATION

•Fixed at the point where it was at the time of fixation.

(7)

COMMONLY USED FIXATIVES

• Mercuric Chloride (HgCl2)

• Potassium Dichromate (K2Cr2O7)

• Chromic Acid (H2CrO4) or Chromium Trioxide (CrO3) • Osmium Tetroxide (OsO4)

• Ethanol (C2H5OH)

• Acetic Acid (CH3COOH)

(8)

Fixing Mixtures

• Flemming’s Weak Fluid • Flemming’s Strong Fluid • Carnoy’s Fluid

(9)
(10)

The Feulgen Reaction

(11)

Squash and Smear Preparation

• Smears are formed when fresh tissue is spread over a slide and no treatment is necessary.

(12)
(13)

Fluorescence in situ hybridisation (FISH)

1) Tissue collection: 2) Fixation:

3) Enzyme digestion 4) Protoplast isolation

5) Hypotonic treatment of protoplasts

6) Cleaning and fixation of the protoplasts 7) Protoplast dropping:

Referanslar

Şimdi indir ( PDF - 13 sayfa - 292.31 KB )

Benzer Belgeler

This is the definition of a derivative

S analytical is the analytical solution and S numerical is the numerical solution. The absolute value is taken to account equally for over and under estimates of the solution.

How fast is the top of the ladder sliding down the wall then? 4

Just when the balloon is 65 ft above the ground, a bicycle moving at a constant rate of 17 ft/sec passes under it.. How fast is the distance between the bicycle and balloon increasing

This regulation is mandatory for social progress. The necessary tool is sociology, that is, positive science.

For this reason, there is a need for science and social science that will reveal the laws of how societies are organized and how minds are shaped.. Societies have gone through

The implementation of this work is done by using a proposed power system with the following considerations

A proposed case study is simulated using Matlab software program in order to obtain the overload case and taking the results of voltage and current in the distribution side,

d) Perishability This is the real-time nature of the product which occurs as a result simultaneous production and consumption

Marketing channel; describes the groups of individuals and companies which are involved in directing the flow and sale of products and services from the provider to the

The XRD will be the secondary science mission on board BeEagleSat, which is developed as one of the double CubeSats for the QB50 project

A CdZnTe based semiconductor X-ray detector (XRD) and its associated readout electronics has been developed by the Space Systems Design and Testing Laboratory of Istanbul

This premise is central to the narratives of the Balkan nationalisms

Svetosavlje views the Serbian church not only as a link with medieval statehood, as does secular nationalism, but as a spiritual force that rises above history and society --

The population of this study is the fourth elementary classes

The aim of this study is to provide developing students’ awareness of mathematics in our lives, helping to connect with science and daily life, realizing