THE ROLE OF Δ
9-THC ON OXIDATIVE STRESS STATUS OF
BRAIN AND CEREBELLUM IN TYPE-2 DIABETES
5th THE INTERNATIONAL CONGRESS ON CELL MEMBRANES AND OXIDATIVE STRESS: FOCUS ON CALCIUM
SIGNALING AND TRP CHANNELS, 9 - 12 SEPTEMBER 2014, ISPARTA-TURKEY
ZEYNEP MINE COSKUNa, SEMA BOLKENTb
aDepartment of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Bilim University, Istanbul, Turkey bDepartment of Medical Biology, Faculty of Cerrahpasa Medicine, Istanbul University, Istanbul, Turkey
INTRODUCTION
MATERIAL AND METHOD
RESULTS
REFERENCES
[1] Ceretta LB et al. Increased oxidative stress and imbalance in antioxidant enzymes in the brains of alloxan-induced diabetic rats. Exp Diabetes Res. 2012;2012:302682.[2] Shinde SN et al. Evaluation of oxidative stress in type 2 diabetes mellitus and follow-up along with vitamin E supplementation. Indian J Clin Biochem 2011; 26: 74-77. [3] Chen J et al. Reactive oxygen species and p38 phosphorylation regulate the protective effect of delta9-tetrahydrocannabinol in the apoptotic response to NMDA. Neurosci Lett 2005; 389: 99-103. [4] Moldzio R et al. Effects of cannabinoids tetrahydrocannabinol, Δ(9)-tetrahydrocannabinolic acid and cannabidiol in MPP+ affected murine mesencephalic cultures. Phytomedicine 2012; 19: 819-824. [5] Sagredo O et al. Neuroprotective effects of phytocannabinoid-based medicines in experimental models of Huntington's disease. J Neurosci Res 2011; 89: 1509-1518.
8-10 week-old Sprague-Dawley rats were divided into 4 groups. Group I: Physiological saline was administered intraperitoneally (i.p) (n=7). Group II: Rats
that are given Δ9-THC for 7 days (3 mg/kg/day) (n=6)
(i.p). Group III: Streptozotocin (STZ, 65 mg/kg) +Nicotinamide (NAD, 85 mg/kg) (n=7) (i.p). Group IV:
Diabetic rats that are given Δ9-THC (3 mg/kg/day) for 7
days (n=7) (i.p). Malondialdehyde (MDA) and reduced glutathione (GSH) levels, superoxide dismutase (SOD) and catalase (CAT) activities were measured in brain and cerebellum tissue samples of rats.
Diabetes Mellitus is a metabolic disorder characterized by hyperglycemia. The hyperglycemia leads to neuronal damage [1]. The development and progression of diabetes mellitus and its complications are derived from increased oxidative damage [2]. Many
studies report that Δ9-THC is protective for neuronal
injury in experimental models and exhibits anti-oxidative action [3-5].
In this study, we aimed to explore the curative effect
on oxidative stress of Δ9THC in the brain and
cerebellum of type-2 diabetic rats.
The results showed that GSH levels of the brain and cerebellum were non-significantly decreased in the diabetic rats as compared with the control group. The GSH levels of the brain and cerebellum were non-significantly
increased in the diabetes+Δ9-THC group when compared with the diabetic group. It was found that the increased
MDA levels of the brain and cerebellum in diabetic rats were decreased by treatment with Δ9-THC. In the diabetic rats
treated with Δ9-THC, the SOD activities of the brain and cerebellum were elevated as compared with diabetes group.
The brain and cerebellum CAT activities were non-significantly increased in the diabetic rats treated with Δ9-THC, as
compared with the diabetes group.
CONCLUSION
In conclusion, it is suggested that Δ
9-THC has curative effects against oxidative stress in type-2 diabetic
rats. Δ
9-THC may be conferred in diabetes treatment with an appropriate dose due to its antioxidant effects.
*Mean ± Standart Error of Mean (SEM), NS: Non-significant
aP<0.01 versus control group; bP<0.05 versus control group; cP<0.01 versus Δ9-THC group; dP<0.01 versus diabetes group; eP<0.05 versus diabetes group
Table: Biochemical parameters in all groups
BRAİN CEREBELLUM Groups GSH* (nmol/mg) MDA* (nmol/mg) SOD* (U/mg) CAT* (U/mg) GSH* (nmol/mg) MDA* (nmol/mg) SOD* (U/mg) CAT* (U/mg) Control (n=7) 50.09 ± 3.07 4.07 ± 0.37 4.16 ± 0.36 9.53 ± 1.16 48.49 ± 4.29 3.69 ± 0.33 7.29 ± 0.38 11.82 ± 0.50 Δ9-THC (n=6) 63.59 ± 9.50 6.41 ± 0.92b 6.05 ± 0.93 10.12 ± 2.01 38.47 ± 4.53 3.51 ± 0.35 5.52 ± 0.58b 7.55 ± 1.16 Diabetes (n=7) 48.06 ± 2.86 6.47 ± 0.80a 4.56 ± 0.35 8.54 ± 1.06 39.78 ± 3.47 4.96 ± 0.21a,c 5.45 ± 0.56a 8.84 ± 1.21 Diabetes + Δ9-THC (n=7) 62.27 ± 9.53 5.45 ± 1.50 5.24 ± 0.69 9.12 ± 1.31 45.47 ± 3.03 3.74 ± 0.33d 6.86 ± 0.51e 8.98 ± 1.65 PANOVA NS NS NS NS NS P<0.05 NS P<0.05
