Rapid fixation of whole organ specimens and attendant problems.

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Rapid ﬁxation of whole organ specimens and attendant problems

Article in Acta medica Okayama · May 1992

DOI: 10.18926/AMO/32649 · Source: PubMed

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Acta Medica Okayama

Volume 46, Issue 2

1992

Article 3

A

PRIL

1992

Rapid fixation of whole organ specimens and

attendant problems.

Behice Durgun-Yucel

∗

Fahri Dere

†

Ahmet H. Yucel

‡

Ozkan Oguz

∗∗

∗_{Cukurova University,} †_{Cukurova University,} ‡_{Cukurova University,} ∗∗_{Cukurova University,}

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Rapid fixation of whole organ specimens and

attendant problems.

∗

Behice Durgun-Yucel, Fahri Dere, Ahmet H. Yucel, and Ozkan Oguz

Abstract

Gross specimens are valuable sources in morphology education. In this study, we investi-gated how the fixation of gross specimens may be accelerated. For this purpose, whole organ specimens from freshly killed rabbits: extremities, kidney, heart, liver, stomach and uterus were fixed in a mercaptoethanol-formaldehyde mixture for 3-3.5h under the following conditions: 1, at room temperature; 2, at gradually increasing temperatures up to 45 degrees C; and 3, at a grad-ually increasing vacuum ranging from 20 kPa to 40 kPa. The results were compared with those of formaldehyde-fixed controls, and the mercaptoethanol-formaldehyde mixture was found to be useful in shortening the fixation time and providing good fixation. Both heat and vacuum enhanced these phenomena.

KEYWORDS: rapid fixation, mercaptoethanol-formaldehyde mixture, aldehyde, whole organ speciments

∗_{PMID: 1575061 [PubMed - indexed for MEDLINE]}

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