The Fifth Conference “ Nuclear Science and Its Application”, 14-17 October 2008
INFLUENCE
OF
IONIZING
RADIATION
ON
GROWTH
OF
MACROMYCETES
A.K. GARAYUSIFOVA, * A.A. RZAYEV. * A.S. ABDULLAYBV. * E.N. SHAMILOV, P.Z. MURADOV
Institute of Microbiology, Azerbaijan National Academy of Sciences *Institute of Radiation Problems, Azerbaijan National Academy of Sciences
Increasing of general background radiation is one of the changes in nature caused by anthropogenic factors. Due to the influence of ionizing radiation cells on living beings they are damaged and in some cases it results in sudden or gradual death. Different approaches are used to prevent this process. One of them is finding and practical applying of radioprotectors.
Therefore the main goal o f this research is to find the range of the dose that stops the growth o f the mushroom Pleurotus Ostreatus and studying the radioprotective properties o f Greek nut (Juglans regia L.) extract added into the nutrient medium.
For incubating o f Pleurotus Ostreatus both standard wort agar (4°B) and wort agar (4°B) containing 0.1 % Greek nut extract were used. Incubation of samples was continued during 10 days at the temperature of 26-28°C. Influence was estimated by the diameter o f the colony (mm) formed by the mushroom.
Samples were irradiated in “K-25” (USSR) installation at dose o f 500, 1000, 1500 and 2000 Gy (60Co, P=0.52 Gy/sec).
During the research it has been defined that 2000 Gy is enough for the mushroom completely to lose its ability to live in standard wort agar. Addition o f 0.1 % Greek nut extract into the wort agar increases the range of the semilethal dose up to 1250-1300 Gy. In this case increasing the dose rang up to the 2000 Gy delays the growth for 7-10 days. But growth in standard wort agar medium without Greek nut extract at the dose 2000 Gy was not observed.
The Fifth Conference “ Nuclear Science and Its Application”, 14-17 October 2008
Recently researches on influence of yarrow
(Achillea
Filipendulina) and saffron (Crocus Sativus L.) extracts cobalt, ammonium and copper rutinate solutions on the ability to live of the mushroom Pleurotus Ostreatus are being conducted.Section IV. Application O f Nuclear Technologies In Industry, Medicine And Agriculture 157
