VERİ SETİ VE MODELİN DEĞİŞKENLERİ

Fotomicrografia de uma distensão de sangue obtido de ratos Wistar tratados com solução salina (NaCl 0.9%) durante 7 dias. As distensões sangüíneas foram preparadas, secadas e coradas pelo método May-Grünwald-Giemsa. As lâminas foram analisadas por microscopia óptica (aumento de 1000x) e as imagens capturadas para análise.

6.5- Efeito in vivo de um extrato de farinha da casca de maracujá na morfologia de hemácias de ratos Wistar.

Fotomicrografia de uma distensão de sangue obtido de ratos Wistar tratados com um extrato de Farinha da casca de maracujá (50mg/kg) durante 7 dias. As distensões sangüíneas foram preparadas, secadas e coradas pelo método May- Grünwald-Giemsa. As lâminas foram analisadas por microscopia óptica (aumento de 1000x) e as imagens capturadas para análise.

6.6- Efeito in vivo de um extrato de farinha da casca de maracujá na biodisponibilidade da radiobiocomplexo pertecnetato de sódio em ratos Wistar.

Orgão %ATI/g Controle P. flavicarpa Cérebro 0,01 ± 0,01 0,01 ± 0,01 Fígado 2,06 ± 0,65 2,18 ± 0,46 Duodeno 0,81 ± 0,13 0,44 ± 0,13* Coração 0,58 ± 0,17 0,57 ± 0,24 Rim 0,75 ± 0,10 0,90 ± 0,07 Baço 0,68 ± 0,10 0,47 ± 0,09* Pulmão 1,09 ± 0,30 1,22 ± 0,03 Estômago 2,84 ± 0,18 4,92 ± 0,08* Pâncreas 0,08 ± 0,01 0,05 ± 0,01* Sangue 1,43 ± 0,07 1,07 ± 0,09* Osso 0,30 ± 0,08 0,20 ± 0,02 Músculo 0,07 ± 0,01 0,10 ± 0,02 Tireóide 5,13 ± 0,67 4,30 ± 0,31 Testículo 0,27 ± 0,08 0,16 ± 0,05

Ratos Wistar tratados com um extrato de Farinha da casca de maracujá (50 mg/kg) durante 7 dias por via oral ou com solução salina 0,9% para o grupo controle. Em seguida, Na99mTcO4 (0,3 mL) foi injetado no animal pelo plexo ocular e os mesmos foram sacrificados após 10 minutos. Os órgãos foram isolados, a radioatividade foi contada e a %ATI/g em cada órgão foi determinada. (*) diferença significante (p<0,05) em relação ao controle.

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Blood constituents labelled with technetium-99m (99mTc) has been used with radiobiocomplexes in several procedures in nuclear medicine. Some natural and sintetic drugs are capable to interfere on the labeling of blood constituents with 99mTc, on the morphology of red blood cells (RBC) and on the biodistribution of radiobiocomplexes. The aim of this study was evaluate the effect of an extract of Passiflora edulis f. flavicarpa on the labeling of blood constituints with 99mTc, on the morphology of RBC and on the biodistribution of the radiopharmaceutical sodium perthecnetate in Wistar rats. On the in vitro studies the Passiflora edulis f. flavicarpa decreased significantly (p<0.05) the %ATI on plasma proteins and on the in vitro morhology of RBC, the passion fruit peel flour altered the shape and the perimeter/área ratio. On the in vivo estudies the extract did not altered the %ATI in blood constituents, and did not altered the shape of RBC. Although, on the biodistribution of the radiobiocomplex sodium perthecnetate (Na99mTcO4) this extract decreased significantly (p<0.05) the uptake in duodenum, spleen, pâncreas and blood, and increased the uptake in stomach. It can be suggested that the effects presented by this extract could be a result of some substances contained in this extract that could alter the binding of 99mTc to plasma proteins, the morphology of RBC and the biodistribution of the radiobiocomplex sodium perthecnetate. This study was multidisciplinary experimental research. It was developed with the contribution of different Departments and Services of the Hospital Universitário Pedro Ernesto of the Universidade Estadual do Rio de Janeiro.