Gelecek ÇalıĢmalar için Öneriler

Conclusões Gerais

Nas células 3T3-L1 foram observados os seguintes eventos:

• A adição de LPS aumentou as concentrações de IL-6, enquanto que a adição das vitaminas

E ou D3 reduziram essas concentrações;

• A adição das vitaminas E ou D3 reduziu a relação IL-6/IL-10.

Os camundongos alimentados com dieta hiperlipídica em relação ao grupo controle tiveram:

• Maior consumo energético;

• Maior conteúdo de gordura da carcaça;

• Maior peso relativo dos tecidos retroperitoneal, epididimal, mesentérico e fígado; • Maior conteúdo de gordura hepática;

• Maiores concentrações hepáticas de TBARS; • Maior expressão gênica de TLR4 e SOD; • Menor expressão gênica de GPx;

• Maior expressão protéica TLR4, TRAF6;

• Menor expressão protéica de catalase, GPx e SOD.

A suplementação de vitamina D3 nos camundongos alimentados com dieta

hiperlipídica promoveu:

• Redução do ganho de massa corporal, independentemente do consumo energético; • Redução do conteúdo de gordura da carcaça;

• Redução do peso relativo dos tecidos retroperitoneal, epididimal, mesentérico e fígado; • Redução das concentrações séricas de glicose e insulina;

• Redução do conteúdo de gordura hepático; • Redução das concentrações hepáticas de TBARS;

• Menor concentração de IL-6 no tecido adiposo epididimal; • Menor relação IL-6/IL-10 no tecido adiposo epididimal; • Redução da expressão gênica de TLR4;

• Aumento da expressão gênica de SOD, catalase e GPx; • Redução na expressão protéica de TLR4, MyD88, TRAF6; • Aumento na expressão protéica de GPx e SOD.

A suplementação de vitamina E nos camundongos alimentados com dieta hiperlipídica promoveu:

• Redução do ganho de massa corporal, independentemente do consumo energético; • Redução do peso relativo dos tecidos retroperitoneal, epididimal, mesentérico e fígado; • Redução das concentrações séricas de glicose;

• Aumento nas concentrações séricas de vitamina E; • Redução do conteúdo de gordura hepático;

• Redução das concentrações hepáticas de TBARS;

• Menor concentração de IL-6 no tecido adiposo epididimal; • Menor relação IL-6/IL-10 no tecido adiposo epididimal; • Redução da expressão gênica de TLR4 e TNF- ;

• Aumento da expressão gênica de GPx;

• Redução na expressão protéica de TLR4, MyD88, TRAF6; • Aumento na expressão protéica de GPx e SOD.

Em resumo, o presente estudo demonstrou que a suplementação de vitamina E ou D3

possui atividade anti-obesidade in vivo. A suplementação de vitamina E ou D3 reduziu a

expressão gênica e protéica de TLR4 e aumentou a expressão de enzimas antioxidantes, vias inflamatória e oxidativa relacionadas ao processo de desenvolvimento de obesidade.

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