TECHNIQUE ON THE HEALING AFTER THE REPAIR AND THE CHRONIC SUPRASPINATUS MUSCLE RUPTURE MODEL FORMED
IN RATS
SUMMARY
Our study was conducted to answer the question of how to make a more effective repair at the tendon-bone junction after chronic massive rotator cuff tear repair. For this purpose, the effects of extracorporeal shock wave therapy and Crimson Duvet augmentation technique on healing were compared. Our study was carried out on 30 adult, Sprague-Dawley rats obtained from Trakya University Faculty of Medicine Experimental Animals and Research Laboratory with the approval of Trakya University Animal Experiments Local Ethics Committee (Annex I).
The rats were divided into 3 groups. A chronic rotator cuff tear model was created for each group. Group I (control group): Only rotator cuff repair was performed. Group II: Rotator cuff repair and extracorporeal shock wave therapy was performed. Group III: Rotator cuff repair was performed using the Crimson Duvet technique.
In the post-operative 2nd week, the subjects in Group II were administered extracorporeal shock wave therapy at 1.0 Bar, 1000 beats, 10 Hz. In the post-operative 4th week, 5 subjects from each group were sacrificed. In the post-operative 6th week,
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extracorporeal shock wave therapy with the same features was applied to the remaining 5 subjects in Group II. All remaining subjects were sacrificed 8 weeks after repair.
Histopathological and immunohistochemical evaluations of the tendon bone junctions of the subjects were performed.
Although the Bonar Scale score was lower in Group III than other groups in histomorphological evaluation, it was found to be more similar to normal tendon structure in terms of tenocyte structure, ground material and vascularity, but a significant difference was found only in terms of vascularity. In the immunohistochemical evaluation, although bone morphogenetic protein-2 and bone morphogenetic protein-7 levels were more expressed in Group III, it was not statistically significant. There was no significant difference between groups in expression of transforming growth factor-1. Although there was an increase in the vascular endothelium showing immunoreactivity with CD 34 antibody in Group II, there was no statistically significant difference.
As a result, Group III using biological augmentason technique seems to be more effective in healing of tendon-bone junction, but no significant result was achieved. It is predicted that a meaningful result can be achieved by repeating the study by reducing the limitations of the study and increasing the number of subjects.
Keywords; Extracorporeal shock wave therapy, Crimson Duvet technique, Tendon bone junction healing, Chronic massive rotator cuff rupture
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