5. TARTIŞMA ve SONUÇ
5.2 Mikroalg Hücrelerinin Lipit Biriktirme Kapasitelerinin Araştırılması
5.2.4 Biriktirilen lipitlerin yağ asidi dağılımları
Damiani vd. (2010) yaptıkları çalışmada; Haematococcus pluvialis mikroalginin denenen tüm koşullarda yağ asidi dağılımının birbirine benzemekle birlikte başlıca bileşenlerin palmitik, stearik, oleik, linoleik, linolenik ve linolelaidik asit olduğunu bulmuşlardır. Doymuş yağ asidi oranı A stres ortamı, B stres ortamı ve kontrol ortamında sırasıyla %30.36, %29.62 ve %27.81 olarak bildirilmiştir. Çalışmada bulunan başlıca yağ asidi metil esterleri olan C16:0 için kontrol, A stres ve B stres ortamlarında sırasıyla %22.49, %18.87, %21.29; C18:0 için %3.15, %7.07, %5.69; C18:1n9c için
%19.36, %18.25, %18.35; C18:2n6t için %6.67, %5.37, %7.57; C18:2n6c için ise
%20.23, %22.06, %22.9 olarak bulunmuştur.
Gao vd. (2010) yaptıkları çalışmada, Chlorella protothecoides mikroalginden elde edilen lipitleri asit bir katalizör varlığında transesterifikasyon reaksiyonuna uğratmışlardır. Hem glukoz hem de sorgum pekmezi içeren ortamda geliştirdikleri hücrelerden ekstrakte edilen lipitlerin transesterifikasyon reaksiyonu sonucunda en fazla oleik, linoleik ve setan asit metil esterlerini bulunmuştur. Bu oranlar glukoz içeren ortamda sırasıyla %53.75, %19.48, %11.34 iken sorgum pekmezi içeren ortamda geliştirilen hücrelerde %66.80, %15.12, %12.66 olarak gözlenmiştir.
Yoo vd. (2010) çalışmada denedikleri her üç mikroalg türü içinde (Botryococcus braunii, Chlorella vulgaris ve Scenedesmus sp.) bulunan yağ asidi metil esterleri C16:0, C16:1, C18:0, C18:1, ve C18:2 olarak bildirilmiştir. Bu yağ asitleri arasından palmitik, oleik ve linoleik asit en fazla yağ asitleriyken, palmitoleik ve stearik asit daha az bulunmuştur. C16:0, C16:1, C18:0, C18:1, ve C18:2 yağ asitleri Botryococcus braunii mikroalginde sırasıyla % 29.5, %3.4, %1.0, %44.9, %21.2; Chlorella vulgaris’de
%24.0, %2.1, %1.3, %24.8, %47.8; Scenedesmus sp. mikroalginde ise %36.3, %4.0,
%2.7, %25.9, %31.1 olarak bildirilmiştir.
99
Gerçekleştirilen çalışmada Synechococcus sp. hücrelerinden elde edilen lipitin doymuşluk oranı %74.5, Cyanobacterium aponinum hücrelerinden elde edilen lipitin
%77.9, Phormidium sp. hücrelerinden elde edilen lipitin ise %84.7 olarak elde edilmiştir. Çalışmada kullanılan her üç siyanobakteri hücre lipitlerinin de başlıca bileşenlerinin C16:0 (palmitik asit) ve C18:0 (stearik asit) olması sebebiyle biyodizel üretiminde önemli hammaddeler olabileceği düşünülmektedir. Her üç siyanobakteri hücresinden de elde edilen lipitlerin doymuş yağ asitlerinden oluşuyor olması, bu lipitlerden üretilecek biyodizelin yüksek setan sayısına ve çok güçlü oksidatif kararlılığa sahip olabileceğini göstermektedir. Bu yüzden uzun süreli depolanması sırasında daha az sorunla karşılaşılacağı düşünülmektedir.
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109 ÖZGEÇMİŞ
Adı Soyadı : Sevgi ERTUĞRUL KARATAY Doğum yeri : Çekerek
Doğum Tarihi : 22. 11. 1980 Medeni Hali : Evli
Yabancı Dili : İngilizce
Eğitim Durumu (Kurum ve Yıl)
Lisans : Ankara Üniversitesi Fen Fakültesi Biyoloji Bölümü (2002)
Yüksek Lisans : Ankara Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı ( 2005)
Doktora : Ankara Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı (2010)
Çalıştığı Kurum :
Ankara Üniversitesi, Biyoloji Bölümü, Araştırma Görevlisi, 2002-
Yayınları (SCI kapsamında)
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