Linfonodos: ___________________________________________________________________ Edema extra-oral: ______________________________________________________________ Edema intra-oral: ______________________________________________________________ Fístula (cutânea ou mucosa): _____________________________________________________
EXAME DO DENTE _____________
EXAME SUBJETIVO
( ) Dor presente ( ) Contínua ( ) Intermitente
( ) Cessa com analgésico ( ) Exacerbada ( ) Frio
( ) Calor ( ) Mastigação ( ) Dor provocada ( ) Frio
( ) Calor ( ) Mastigação ( ) Dor ausente ( ) Sem antecedentes
( ) Com antecedentes
EXAME OBJETIVO
Teste de percussão vertical: Teste de percussão horizontal:
( ) Dor presente ( ) Dor ausente ( ) Dor presente ( ) Dor ausente
Mobilidade: Teste de palpação:
( ) Presente ( ) Ausente ( ) Dor presente ( ) Dor ausente
Coroa:
( ) Íntegra ( ) Com exposição pulpar
( ) Restaurada ( ) Parcialmente destruída
( ) Com lesão cariosa ( ) Totalmente destruída
Diagnóstico pulpar: _________________________________________________________ EXAME RADIOGRÁFICO Lesão periapical: Tamanho: _____________________ EXAME HISTOPATOLÓGICO Diagnóstico da lesão: ________________________________________________________________
ANEXO C – Soluções e reagentes
A – Soluções utilizadas na eletroforese em gel de poliacrilamida 6,5%
a) Acrilamida 30% • Acrilamida 29g • Bis-acrilamida 1g • H2O q.s.p. 100ml b) TBE 20X • Tris 121g • Ácido bórico 61,7g • EDTA 7,44g • H2O q.s.p. 1000ml c) TBE 10X • TBE 20X 500ml • H2O q.s.p. 1000ml d) TBE 1X • TBE 50X 50ml • H2O q.s.p. 1000ml
e) Confecção do gel de poliacrilamida
• Acrilamida 30% 1,079ml
• TBE 10X 0,65ml
• Água destilada 3,22ml
• TEMED 4,00µl
B – Soluções utilizadas na coloração do gel de poliacrilamida pela prata a) Solução de prata (estoque)
• Nitrato de prata 20,38 g
• Água destilada q.s.p. 1.000 ml
b) Solução de prata (uso)
• Solução de estoque 8 ml • Água destilada 100 ml • Formaldeído 37% 150 µl c) Revelador • Carbonato de sódio 2,97 g • Água destilada q.s.p. 100 ml • Formaldeído 37% 150µl • Tiossulfato de sódio 10mg/ml 40 µl d) Solução Fixadora
• Ácido acético glacial 100 ml
• Água destilada q.s.p. 1.000 ml
e) Solução de Tiossulfato de sódio
• Tiossulfato de sódio 10 mg
Anexo D – Dados clínicos coletados Código Sintomatologia Fístula mucosa Resto radicular Diagnóstico histopatológico HCMV EBV Tamanho radiográfico da lesão (mm) LP02 Assintomático Não Sim cisto periapical - + 04 x 04 LP03 Sintomático Não Sim
granuloma
periapical - + 02 x 06 LP04 Assintomático Não Sim
granuloma
periapical - + 03 x 07 LP05 Assintomático Não Sim
granuloma
periapical - - 04 x 05 LP06 Assintomático Não Sim
granuloma
periapical - - 03 x 05 LP07 Assintomático Sim Sim cisto periapical - + 05 x 06 LP08 Sintomático Sim Sim
granuloma
periapical - - 04 x 08 LP09 Sintomático Não Sim cisto periapical - + 03 x 03 LP11 Assintomático Não Sim cisto periapical - + 01 x 07 LP12 Assintomático Não Não cisto periapical - + 04 x 04 LP13 Sintomático Não Não
granuloma
periapical - + 03 x 04 LP14 Sintomático Não Não
granuloma
periapical - + 03 x 05 LP15 Assintomático Não Não
granuloma
periapical - + 05 x 07 LP16 Assintomático Não Sim cisto periapical - + 03 x 07 LP17 Sintomático Não Não cisto periapical - + 04 x 05 LP18 Assintomático Não Sim cisto periapical - + 03 x 07 LP20 Assintomático Não Sim
granuloma
periapical - + 03 x 05 LP21 Assintomático Não Não
granuloma
periapical - + 10 x 12 LP22 Sintomático Não Sim
granuloma
periapical - - 04 x 12 LP23 Sintomático Não Sim cisto periapical + + 04 x 08 LP24 Assintomático Não Sim cisto periapical - + 02 x 03 LP25 Sintomático Não Não
granuloma
periapical - + 04 x 05 LP26 Sintomático Não Não
granuloma
periapical - + 04 x 08 LP27 Assintomático Não Não
granuloma
periapical - + 03 x 04 LP28 Assintomático Não Sim
granuloma
periapical - + 05 x 06 LP29 Assintomático Não Não cisto periapical - + 06 x 12 LP30 Assintomático Não sim
granuloma
Código Sintomatologia Fístula mucosa Resto radicular Diagnóstico histopatológico HCMV EBV Tamanho radiográfico da lesão (mm) LP31 Assintomático Não sim
granuloma
periapical - + 06 x 07 LP32 Assintomático Não não cisto periapical - + 02 x 03 LP33 Sintomático Não sim
granuloma
periapical - + 02 x 08 LP34 Assintomático Não não
granuloma
periapical - + 02 x 06 LP35 Sintomático Não não
granuloma
periapical - - 03 x 06 LP36 Sintomático Não sim
granuloma
periapical - - 04 x 04 LP37 Sintomático Não sim cisto periapical - + 03 x 10 LP38 Assintomático Não sim
granuloma
periapical - + 08 x 08 LP39 Assintomático Não sim
granuloma
periapical - + 04 x 08 LP40 Assintomático Não sim
granuloma
periapical - + 02 x 02 LP41 Assintomático Não sim
granuloma
periapical - + 04 x 05 LP42 Assintomático Não sim
granuloma
periapical - + 08 x 09 LP43 Assintomático Não sim
granuloma
periapical - + 02 x 05 LP44 Sintomático Sim não
granuloma
periapical - + 03 x 04 LP45 Sintomático Não não
granuloma
periapical - - 02 x 04 LP46 Sintomático Não sim
granuloma
periapical - + 03 x 06 LP47 Assintomático Não sim cisto periapical - + 09 x 10 LP48 Assintomático Não não
granuloma
periapical - + 03 x 05 LP49 Sintomático Não não
granuloma
periapical - + 01 x 02 LP50 Sintomático Não sim
granuloma
periapical - + 02 x 04 LP51 Assintomático Não não
granuloma
periapical - + 02 x 05 LP52 Assintomático Não sim cisto periapical - + 05 x 05 LP53 Assintomático Não não
granuloma
periapical - + 03 x 03 LP54 Sintomático Não sim
granuloma
periapical - + 01 x 03 LP55 Sintomático Não sim
granuloma
periapical - - 02 x 03 LP56 Assintomático Não sim
granuloma
Código Sintomatologia Fístula mucosa Resto radicular Diagnóstico histopatológico HCMV EBV Tamanho radiográfico da lesão (mm) LP57 Sintomático Não não
granuloma
periapical - + 04 x 05 LP58 Sintomático Não não
granuloma
periapical - - 02 x 03 LP59 Assintomático Não não
granuloma
periapical - - 01 x 04 LP60 Assintomático Não sim
granuloma
periapical - - 03 x 05 LP61 Assintomático Sim não
granuloma
periapical - + 04 x 09 LP62 Sintomático Sim sim
granuloma
periapical - + 03 x 04 LP63 Assintomático Não sim
granuloma
periapical - + 03 x 06 LP64 Assintomático Não sim cisto periapical - + 03 x 08 LP65 Assintomático Não sim cisto periapical - + 08 x 10 LP66 Assintomático Não sim
granuloma
periapical - + 05 x 10 LP67 Assintomático Não sim cisto periapical - + 03 x 03 LP68 Assintomático Não sim
granuloma
periapical + + 04 x 06 LP70 Assintomático Não sim
granuloma
periapical - + 03 x 03 LP71 Assintomático Não sim
granuloma
periapical - + 03 x 05 LP72 Sintomático Não sim
granuloma
periapical - + 03 x 04 LP73 Assintomático Não não cisto periapical + + 05 x 09 LP74 Assintomático Não sim
granuloma
periapical - - 03 x 07 LP75 Assintomático Não não
granuloma
periapical - + 03 x 06 LP76 Assintomático Não não
granuloma
periapical - + 04 x 06 LP77 Assintomático Não sim
granuloma
periapical - + 06 x 08 LP78 Assintomático Não sim
granuloma
periapical - + 04 x 04 LP79 Assintomático Não não
granuloma
periapical - - 02 x 03 LP80 Sintomático Não sim
granuloma
periapical - + 04 x 03 LP81 Assintomático Não não
granuloma
periapical - - 06 x 12 LP82 Assintomático Não não
granuloma
periapical - + 02 x 06 LP83 Sintomático Não não
granuloma
Paciente Idade Gênero ARS 24 feminino ASP 21 feminino CDM 23 feminino CFJM 28 feminino CO 37 masculino CMCS 36 feminino CLF 31 feminino CF 31 masculino DAF 65 masculino DGF 58 feminino EPB 44 masculino EGSP 27 feminino ERS 30 feminino EFS 42 feminino FALF 41 masculino FRB 14 masculino GPUC 67 feminino GTS 53 masculino GMC 23 feminino IRS 47 feminino JSC 33 feminino JSL 18 feminino JCPC 26 feminino JMS 53 feminino LCM 35 feminino LGC 28 feminino LFS 28 masculino COM 45 masculino MRF 38 feminino MGFT 39 masculino MHO 35 feminino MAO 50 feminino MCDD 43 feminino MPCJ 49 feminino MFC 52 feminino
Paciente Idade Gênero MEF 35 feminino MLM 42 feminino MCA 40 feminino MGS 28 feminino MAS 44 masculino MA 21 masculino NMSA 44 feminino NGL 55 feminino PCDA 22 masculino RSM 31 masculino RR 32 feminino RCH 47 feminino SRAF 25 masculino SMF 22 feminino SS 61 masculino SSC 31 feminino SD 39 masculino SOS 42 feminino SAR 33 feminino SCC 39 feminino VLPM 51 feminino VMS 68 masculino VPM 30 feminino VAS 24 feminino VFS 25 feminino VPS 30 feminino
Anexo E – Normas para publicação – International Endodontic Journal
Disponível em http://www.blackwellpublishing.com – acesso em 30/12/2006. International Endodontic Journal
The Official Journal of the British Endodontic Society, the European Society of Endodontology, the Flemish Society of Endodontology, the Irish Endodontic Society and the Portuguese Society of Endodontology
Edited by: Paul Dummer Print ISSN: 0143-2885 Online ISSN: 1365-2591 Frequency: Monthly
Current Volume: 39 / 2006
ISI Journal Citation Reports® Ranking: 2005: 21/49 (Dentistry, Oral Surgery & Medicine)
Impact Factor: 1.606 Top Author Guidelines Title Page
The title page should bear:
(i) Title, which should be concise as well as descriptive (ii) Initial(s) and last (family) name of each author
(iii) Name and address of department, hospital or institution to which work should be attributed
(iv) Running title (no more than 30 letters and spaces) (v) No more than six keywords (in alphabetical order)
(vi) Name, full postal address, telephone, fax number and e-mail address of author responsible for correspondence.
Abstract:
Original Scientific Articles must have a structured abstract of not more than 250 words giving details of what was done using the following structure:
Aim Give a clear statement of the main aim of the study and the main hypothesis tested, if any.
Methodology Describe the methods adopted including, as appropriate, the design of the study, the setting, entry requirements for subjects, use of materials, outcome measures and statistical tests.
Results Give the main results of the study, including the outcome of any statistical analysis.
Conclusions State the primary conclusions of the study and their implications. Suggest areas for further research, if appropriate.
Main Text
Original Scientific Articles
Introduction: should be focused, outlining the historical or logical origins of the study and gaps in knowledge; exhaustive literature reviews are not appropriate. It should close with the explicit statement of the specific aims of the investigation, or hypothesis to be tested.
Materials and Methods: must contain sufficient detail such that, in combination with the references cited, all clinical trials and experiments reported can be fully reproduced. Clinical trials should be reported using the CONSORT guidelines available at http://www.consort-statement.org/newene.htm. A CONSORT checklist should also be included in the submission material (http://www.consort- statement.org/newene.htm#checklist).
(i) Experimental Subjects: Experimentation involving human subjects will only be published if such research has been conducted in full accordance with ethical principles, including the World Medical Association Declaration of Helsinki (version VI, 2002 http://www.wma.net/e/policy/b3.htm) and the additional requirements, if any, of the country where the research has been carried out. Manuscripts must be accompanied by a statement that the experiments were undertaken with the understanding and written consent of each subject and according to the above mentioned principles. A statement regarding the fact that the study has been independently reviewed and approved by an ethical board should also be included in the manuscript.
All studies using human or animal subjects should include an explicit statement in the Material and Methods section identifying the review and ethics committee approval for each study, if applicable.
Editors reserve the right to reject papers if there is doubt as to whether appropriate procedures have been used.
(ii) Suppliers: Suppliers of materials should be named and their location (Company, town/city, state, country) included.
Results: should present the observations with minimal reference to earlier literature or to possible interpretations. Data should not be duplicated in Tables and Figures.
Discussion: may usefully start with a brief summary of the major findings, but repetition of parts of the abstract or of the results section should be avoided. The Discussion section should progress with a review of the methodology before discussing the results in light of previous work in the field. The Discussion should end with a brief conclusion and a comment on the potential clinical relevance of the findings. Statements and interpretation of the data should be appropriately supported by original references.
Conclusions: should contain a summary of the findings.
Acknowledgements: The International Endodontic Journal requires that all sources of institutional, private and corporate financial support for the work within the manuscript must be fully acknowledged, and any potential conflicts of interest noted. Grant or contribution numbers may be acknowledged, and principal grant holders should be listed. Acknowledgments should be brief and should not include thanks to anonymous referees and editors.
References: It is the policy of the Journal to encourage reference to the original papers rather than to literature reviews. Authors should therefore keep citations of reviews to the absolute minimum.
In the text: single or double authors should be acknowledged together with the year of publication, e.g. (Pitt Ford & Roberts 1990). If more than two authors the first author followed by et al. is sufficient, e.g. (Tobias et al. 1991).
Reference list: All references should be brought together at the end of the paper in alphabetical order and should be in the following form.
(i) Names and initials of up to six authors. When there are seven or more, list the first three and add et al.
(ii) Year of publication in parentheses
(iii) Full title of paper followed by a full stop (.) (iv) Title of journal in full (in italics)
(v) Volume number (bold) followed by a comma (,) (vi) First and last pages
Electronic Figures and Tables
It is essential that all artwork is provided in electronic format. Please save vector graphics (e.g. line artwork) in Encapsulated Post-script Format (EPS) and bitmap files (e.g. half-tones) in Tagged Image Format (TIFF). Detailed information on our
digital illustration standards is available at
All figures should be planned to fit within either 1 column width (8.0 cm), 1.5 column widths (13.0 cm) or 2 column widths (17.0 cm), and must be suitable for photocopy reproduction from the printed version of the manuscript. Lettering on figures should be in a clear, sans serif typeface (e.g. Helvetica); if possible, the same typeface should be used for all figures in a paper. After reduction for publication, upper-case text and numbers should be at least 1.5-2.0 mm high (10 point Helvetica). After reduction, symbols should be at least 2.0-3.0 mm high (10 point). All half-tone photographs should be submitted at final reproduction size. In general, multi-part figures should be arranged as they would appear in the final version. Reduction to the scale that will be used on the page is not necessary, but any special requirements (such as the separation distance of stereo pairs) should be clearly specified.
Unnecessary figures and parts (panels) of figures should be avoided: data presented in small tables or histograms, for instance, can generally be stated briefly in the text instead. Figures should not contain more than one panel unless the parts are logically connected; each panel of a multipart figure should be sized so that the whole figure can be reduced by the same amount and reproduced on the printed page at the smallest size at which essential details are visible.
Figures should be on a white background, and should avoid excessive boxing, unnecessary colour, shading and/or decorative effects (e.g. 3-dimensional skyscraper histograms) and highly pixelated computer drawings. The vertical axis of histograms should not be truncated to exaggerate small differences. The line spacing should be wide enough to remain clear on reduction to the minimum acceptable printed size.
Figures divided into parts should be labelled with a lower-case, boldface, roman letter, a, b, and so on, in the same typesize as used elsewhere in the figure. Lettering in figures should be in lower-case type, with the first letter capitalized. Units should have a single space between the number and the unit, and follow SI nomenclature or the nomenclature common to a particular field. Thousands should be separated by a thin space (1 000). Unusual units or abbreviations should be spelled out in full or defined in the legend. Scale bars should be used rather than magnification factors, with the length of the bar defined in the legend rather than on the bar itself. In general, visual cues (on the figures themselves) are preferred to verbal explanations in the legend (e.g. broken line, open red triangles etc.)
Figure legends: should begin with a brief title for the whole figure and continue with a short description of each panel and the symbols used; they should not contain any details of methods.
Tables: should be double-spaced with no vertical rulings, with a single bold ruling beneath the column titles. Units of measurements must be included in the column title.
Hard copies of all figures and tables are required when the manuscript is ready for publication. These will be requested by the Editor when required. Each Figure copy should be marked on the reverse with the figure number and the corresponding author's name.
Abbreviations
The International Endodontic Journal adheres to the conventions outlined in Units, Symbols and Abbreviations: A Guide for Medical and Scientific Editors and Authors. When non-standard terms appearing 3 or more times in the manuscript are to be abbreviated, they should be written out completely in the text when first used with the abbreviation in parenthesis.
Permissions
If all or part of previously published illustrations are to be used, permission must be obtained from the copyright holder concerned. This is the responsibility of the authors before submission.
Anexo F – Figuras
FIGURA 3 – Imagens radiográficas de dentes associados a radioluscências periapicais sugestivas de lesões de origem endodôntica