3.2. Anadolu Toplumlarında Ağırlık Ölçüleri
3.2.3 Anadolu’da Hititler Dönemi Ağırlık Ölçüleri
A caracterização morfológica detalhada da embriogênese somática em B.
brizantha a partir de sementes maduras foi demonstrada.
Foi obtido um sistema eficiente para micropropagação a longo prazo de
Brachiaria.
Um sistema alternativo para embriogênese somática de sementes foi obtido usando segmentos basais de plantas cultivadas in vitro.
Foi estabelecida uma metodologia para obtenção, multiplicação e regeneração de suspensões celulares de B. brizantha.
Foi obtido um sistema de expressão transiente de genes para testar vetores e construções para gramíneas via biobalística usando sementes maduras.
Calos embriogênicos bombardeados originaram plântulas resistentes a higromicina, tendo sido confirmada a presença do gene hptII por PCR, sem a presença do gene gus.
Foi estabelecido um sistema de agroinfiltração eficiente, simples, de baixo custo e rápido para expressão transiente de genes para as cultivares Primavera de
Oryza sativa e Marandu de B. brizantha.
Suspensões celulares de B. brizantha são suscetíveis à transformação genética por Agrobacterium tumefaciens, e foram transformadas pelas cepas EHA105 e LBA4404, pela primeira vez. Unidades embriogênicas resistentes a higromicina e expressando estavelmente o gene gus foram obtidas.
A higromicina parece estar impedindo o desenvolvimento de proembriões resistentes ao antibiótico.
A cultivar Primavera de Oryza sativa é recalcitrante à cultura de tecidos. O sistema de embriogênese somática via unidades embriogênicas foi caracterizado, e demonstrou ser ineficiente na regeneração, sendo necessário o aperfeiçoamento da metodologia.
A linhagem EHA105 é capaz de transformar células de unidades embriogênicas da cultivar Primavera de Oryza sativa e a expressão da proteína marcadora GFP foi detectada.
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