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 It is the oxidation reaction of the anomeric

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(1)

XII.WEEK

(2)

12th WEEK

 Reduction of carbohydrates; picric acid test, qualitative benedict test

 Test observations and results report

(3)

 It is the oxidation reaction of the anomeric

carbon of the monosaccharides containing

free semi acetal hydroxyls to the carboxylic

acid.

(4)

 During this reaction, the sugar acts as a

reducing agent; Ag2 + removes ions such as

Hg2 +, Bi3 +, Cu2 +; such sugars are defined

as reducing sugars.

(5)

 Reductive sugar is a type of sugar that forms

aldehyde or ketone in basic solution. Such

sugars, for example, act as reductants in the

Maillard reaction or the Benedict reaction.

(6)

 Reducing sugars include fructose, glucose,

lactose, arabinose, and maltose.The ketose

monosaccharides, which are ketone groups,

and the aldehydic group are called

aldoses. Sucrose is not a reducing sugar.

(7)

Picric acid experiment

This test is based on the conversion of reducing sugars to colored picric acid.

https://koubiyokimyalab.wordpress.com/tag/pikrik asit/

(8)

Qualitative Benedict ezperiment

 It is understood by the Benedict assay whether the solution known to be a carbohydrate is reductive. With this experiment, the presence of sugar in the urine can be determined and its concentration can be determined semiquantitatively.

 Carbohydrates bearing the free aldehyde or ketone group (carboxyl group) reduce the copper ions from + 2 to + 1 due to the effect of heat in the alkaline environment and discoloration is observed. Because all of the monosaccharides have a free carbonyl group, they give a positive result in this experiment.

https://ameliabiochemistry.wordpress.com/tag/fun/

(9)

 The students will be able to apply the knowledge acquired during the previous week.

 In this context, students will be organized into groups of three before the study.

 Each group will be asked to perform picric acid and

qualitative benedict experiments.

(10)

 All consumables, chemicals, laboratory equipment, especially glassware, and machinery and equipment will be provided as required.

 It will be reminded that each student will

be responsible for the issues given to all

other groups within the application.

(11)

 Students will be asked to report their

observations and results within the scope

of the application to the test report

immediately given to them at the end of

their applications.

(12)

 Students will be asked to remove any material they use, and if necessary, they will be expected to help with cleaning.

 Students will be allowed to leave after

laboratory safety and cleanliness is

ensured.

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