Ankara Üniversitesi, Veteriner FakültesiReprodüksiyon ve Suni Tohumlama Anabilim Dalı Timing ?

Timing ?

KORAY TEKIN

Ankara Üniversitesi, Veteriner Fakültesi

Reprodüksiyon ve Suni Tohumlama Anabilim Dalı

Fertile period

Fertilisation period is the time that oocytes are fertilisable within the female reproductive tract 

• Mating trigger ovulation,

• Mature secondary oocyte is ovulated and this may survive for two to four days after ovulation

• Fertilisation period two to five days after mating;

• İncreasing progesterone concentrations close the cervix.

• Sperm must introduced at mating

• For AI, introduce sperm within days 3 to 4 after injection of drugs or mechanical stimulation of the vagina to cause induction of ovulation.

Gary C. W. England, and Marco Russo In Practice 2014;36:249-254

Determining time for insemination

• Queens spontaneously cycling, but not ovulating,

• Return to pro-oestrus approximately every three weeks.

• Ovulation induced by mechanical stimulation or GnRH (25 µ/queen),

hCG (100 iu/queen) on day 2 or 3 of oestrus.

• AI is best performed on days 3 and 4 after the

stimulation

Artificial insemination

technique

• Ejaculate is deposited into the cranial vagina

• Interestingly, semen flow into the uterus in dorsal recumbency and the table tilted 30 degrees.

• Transcervical uterine insemination in the queen is problematic

«vagina is narrow»

• Furthermore, the cervix is positioned on the dorsal wall of the vagina and the cervix is angled craniodorsally, making

directing the catheter through the cervical os technically

difficult.

Number of inseminations

• A single insemination on day 3 or 4

(ie, on day 2 or 3 after ovulation).

• Sedate with alpha

 adrenoreceptor agonist

• Two inseminations should be performed on days 2 and 3 after ovulation when

cryopreserved semen is used.