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Among them, 9.beta.-hydroxyl derivative is being reported for the first time.

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• 系統編號 RC8906-0118

• 計畫中文名稱 Isoteviol 的生化轉換

• 計畫英文名稱 Biotransformations of Isosteviol

• 主管機關 行政院國家科學委員會 • 計畫編號 NSC88-2314-B038-147

• 執行機構 台北醫學院藥學系

• 本期期間 8711 ~ 8807

• 報告頁數 0 頁 • 使用語言 英文

• 研究人員 林淑娟 Lin, Shwu-Jiuan

• 中文關鍵字 生物轉換;穀胱甘?硫轉換?

• 英文關鍵字 Biotransformation;Glutathione transferase;Isosteviol

• 中文摘要

Isosteviol 是由酸水解 Stevioside 所得到的產物,具有潛在的降血糖及降血壓效果。經由 Cunninghamella elegans, Cunninghamella blakesleeana 及 Cunninghamella bainieri 進行微生物轉換後,新的氫氧基被立體專一的引入 Isosteviol 的 7.alpha.-,7.beta.-,9.beta.-,12.beta.-的位置。分離得到的代謝物經由 1D,2D NMR 及 HRFABMS 的光譜分析以決定其構造。

• 英文摘要

Microbial transformation of isosteviol, using Cunninghamella blakesleeana, Cunninghamella elegans and Cunninghamella bainieri, resulted in the isolation of four microbial metabolites that were characterized on the basis of 1D and 2D NMR spectroscopy, and HRFABMS. The metabolites were identified as ent-7.alpha.-hydroxy-16-ketobeyeran-19-oic acid, ent-9.alpha.-hydroxy-16-

ketobeyeran-19-oic acid, ent-12.alpha.-hydroxy-16-keto-beyeran-19-oic acid, and ent-7.beta.-hydroxy-16-ketobeyeran-19-oic acid.

Among them, 9.beta.-hydroxyl derivative is being reported for the first time.

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