TLC-SPECTROPHOTOMETRIC DETERMINATION OF VASICINE ALKALOID FROM MARKETED FORMULATION OF ADHATODA VASICA NEES.

(1)

T

L

C-Spectrop

hotometric Determination of Vasicine Alkaloid

from

Marke

t

ed

Formulation of Adhatoda vasica nees

B.V. GAITONDE, UUlAS V. BHAT

Phytochemistry Laboratory, Goa College of Pharmacy, Panaji -Goa 403001, India

ADllATODA VAS/CA NEES PREPARATLARINDAKİ VASICINE ALKALOİDİ

DÜZEYLERİNİN İNCE TABAKA KROMATOGRAFİSl VE SPEKTROFOTOMETRl

İLE BELıRTİMİ Özet

Piyasada satılan bazı şurup preparatıannda terapötik etkiye sahip etkin madde içeriğinin miktan

belirtilinemektedir. Adulsa (Adlıatada vasica) ekstraktı içeren böyle bir preparatta bulunan vazisin

düzeyleri araştırıldı. Bu araştırma, böyle bir preparat içerisindeki etkin madde miktarının tTK -spektrofotometrik yöntemle tesbit edilebileceğini göstermektedir.

Yapılan literatür araştırmasında benzer bir çalışmaya rastlanmadt.

Summary

Ayurvedic syrup preparations saId in the market do not mention the amount of active ingredient behaving therapeutic effecL Such one preparation containing extract of Adulsa (Adhatada vasica) was

analysed for alkaloid vasicine content. The present work deals with finding out the actual amount of active ingredient present therein by a TLC -spectrophotometric method. Literature survey does not

reveal any work on such prodUCL

Keywords : Ayurvedic syrup -Adlıatada vasica -TLC -Spectrophotometry

Literature Survey

Bhalla

et

al

(1) estimated vasicine ( 1,2,3,9 -

Tetrahydropyrrolo[

2,1 -

b ]quinazolin

-3 -

ol )

from

Adhatoda vasica

Nees, which

were extracted with 90

%

ethanol

and extract

was acidified,

shaken

with chloroform.

The acidic aqueous

extract was adjusted to

pH

8.5 with

ammonia and

extracted

with

chloroform

to

afford

an alkaloid

mixture, containing

0.59 %

alkaloids. Vasicinone and vasicine

were

separated

on column chromatography

us

ing silica gel column

and

chloroform-methanol and ethyl-acetate

(8:2:1)

as

a

mo

b

ile

phase and

then quantitated

spectrophotometrically

at

283 nm and

285 nm respecti

vely

af ter purification

on

TLC.

Adlf Tıp Derg., 4, 15 -17 (1988)

ADL

İ TIP DERGİSİ

Journal of Forensic Medicine

(2)

16 R.V. GAITONDE. li, llHAT

Brain and Thapa

(2) use.d a high performance liquid chromatographic method [or

quinazoline alkaloids vasicine and vasicinone from

adhatoda vasica

Nees. The

compounds were separated on a octadecyl-silica 5 Ilm

colunın

eluted

with

nıethanol

CH

ı

Cl

1

-

HCl0

4

(50:50:0.001)

at

i

mL/min

and UV

SPCCITophoıometric determiııation

was done at 300 nm.

Experimental

The labcl daim on the symp preparation. eadı 100 mL contains: Adul,a (Adooloda va.<ica) exL eq. to - 4 g.

Preparaıion of Tesı So/wion

Lo mL (if synıp sOlUlion was ıaken from above preparation containing Adu.lsa extract and was extracted with 50 mL of aınmonİacal solution of chlolOform. Same process was repeated Lwice with 20 mL of aliquots each time. All separated chlorofonn layers were collected in a 100 mL volumeııie flask and diluıed with chlorofomı.

Preparaıion oj Slandard Soll4Jion

Chromoplaıes of 20 x 20 cm size were prepared wiUı silica gel G of thickness 500 Jlm and then

activated at ıos-

-

110' C for one hour. Each three actlvated chromoplaıe. were taken and sıreaıd

using 0.25. 0.50 and 0.75 mL of test and standard soluüons. The plates were developed in a satmaied

developing chamber using chlorofonn-mcthanol-ıınımonia (50:50:1) as mobile phasc. The platcs wcre

ron to ıl 10 cm which tcck 35 min. Visualizaıion was done by iodine vapours and eonfirnıed by

Dragendorffs reagen! for alkaloids. For the purpose of serapping, a reference plate under the same concliıiom was prepared and then knowing Rf value scrapping was done. The Rf value for alkaloid vasicine was 0,45. The corresponding band was scrapped out and analysed by Shimadzu UV 240/

Visible Spectrophotometer at 285 nm usıng water as medium.

Recovery Experiment

10 mL of symp solution was ıaken. ıo iı 10 mg of pııre alkoloid vasicine was added. From this admınure the quantıty of contem equivalent to LO mL of synıp was removed and extract.ed wiıh

ammomacal solution of chloroform, and then analysed by the above proposed method. The percentage

recovery of alkaloid vasiciııe was ıhus obtained. Further statisttea] evaluaıion indicated the precisioıı