SIMULTANEOUS DETERMINATION OF PHENCYCLIDINE AND KETAMINE BY GAS CHROMATOGRAPHY AND ELECTRON IMPACT MASS SPECTROMETRY

Simultaneous Determination of Phencyclid

i

ne and

Ket

a

m

ine by Gas Chromatography and

Elec

tron

Im

pact

Mass S

p

ectrometry

P. KINTZ, P. MANGIN

Institut de Mectecine Legale, Strasbourg, Cedex, France

GAZ KROMATOGRAFt VE KUTLE SPEKTROMETRtsl iLE FENStKLIDiN

VE KETAMiNiN BiRARADA BELIRTiMi

()zct

Insan viicut StVtSI ve organlarmda birarada bulunan fensiklidin ile ketaminin duyarlt, ozgiil vc miktar b

e-lirtimine olanak saglayan bir yontemle bee-lirtimine ~alt§ildl.lyon yakalaYlcl dedektor (lTD) taktlt kapiler ko· lonlu gaz kromatografmdan yararlamldl. Enjeksiyon sistemi splitless konumunda kullanildt. ITD 70 eV, iyon kaynak slcakltgl 200·220"C arasmda ve m/z araltgl 40·500 amu olacak §ekilde <;:all§tJnldt. Dctcktoriin elektron

I;ogaltlcl voltajl !850V'a ayarlandt. Adl ge~en maddeler ve il; standart (SKF 525 A) bazik <;:ekitleme ve geri

I;ekitleme i§lemleriylc aynldtktan soma, son liekit, 25 m B.P.! kapiler kolona uygulandl. Fensiklidin, keta·

min ve i~ standart i~in kullamlan m/z degerleri slraslyla 200, 180 ve 86 olarak belirlendi. Fensiklidin ve ke·

taminin belirtilcn deney ko§ullannda plazmada saptanabilen en dii§iik diizeyleri 0.5 ve 0.3 ng/ml 'dir. Aym

deney serisi i~erisinde elde edilen varyasyon katslYiSl % 6'dan kii~iiktiir.

Summary

The method presented describes a sensitlve and specific quantitative assay for the simultaneous

dctenn ination of phencyclidine and ketaminc in human fluids and tissues. Drugs and an internal standard, SKF

525 A, are isolated by a basic extraction and back·extraction process. The final extract is separated on a 25 m

capillary column B.P. I and drugs are detected by selected ion monitoring, at m/z 200, 180 and 86 for

phencyclidine, ketamine and the internal standard, respectively. The minimum detectable quantities in plasma

are 0.5 and 0.3 ng/ml, for phencyclidine and ketamine, respectively. Coefficients of variation for within·run

data were less than 6%.

Key words: Phencyclidine. Ketamine - Gas chromatography. Mass chromatography. Determination

Adli TIp Derg., 6, 199·202 (1990)

ADL

İ TIP DERGİSİ

Journal of Forensic Medicine

Chemical

was PH)etl:ed

Adli Tıp Dergisi 1990; 6(3-4): 199-202

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268-269.

Dr. P. Kintz

11,

67085

Cedex

France

Adli Tıp Dergisi 1990; 6(3-4): 199-202