Çiğdem İçhedef, Serap Teksöz, Perihan Ünak, F. Zümrüt Biber Müftüler, E.İlker Medine
Ege University, Institute of Nuclear Sciences, Department of Nuclear Applications
Amino Acids
D-Penicillamine
Radiopharmaceuticals
Radiolabeling
Quality Controls
Results
Conclusion
Radiolabeling of D-PA with
Tc-99m
Quality Control Studies
+ 1 0 0 µ L D - P A s o l u t i o n ( f r o m f r e s h l y p r e p a r e d ) 9 9 m T c - D - P A 1 N N a O H p H = 7 1 0 0 µ L 9 9 m T c O 4- (55.5 MBq) at 80oC, 20 min. 1 mg D-PA / 1 mL H2O + 1 mg SnCl 2 / 1 mL H2O
TLRC Diagram of Tc-99m Rf: 0.91 TLRC Diagram of Reduced Tc-99m Rf: 0.04 TLRC Diagram of 99mTc-D-PA Rf:0.80 Labeling Yield: 98.72±0.64 %
HPLC Chromatograms of 99mTcO
4- and 99mTc-D-PA
(Blue line belongs to 99mTc-D-PA and green line is 99mTcO 4- )
To explore the biological behavior of the labeled compound in definite tissues.
The protocol was approved by Institutional Animal Review Committee.
RATS;
24 weeks of age
110 - 150 g of weight range
Injection;
0.2 mL volume contains 0.05 mg 99mTc-D-PA per rat was
