Microbiological and Nutrient Analysis of Spiriluna platensis Produced in Poultry Manure Enriched Medium

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ANIMAL NUTRITION

10th_{International Animal Science Conference 25-27 October 2018-Antalya, Turkey}

Web : www.animalscienceconference.org Phone: +90 352 606 01 63 e-mail: zooteknikongresi@gmail.com

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Microbiological and Nutrient Analysis of Spiriluna platensis Produced in Poultry Manure Enriched Medium.

M. Tufan1_{İ. Polat Açık}1_{, N. Toy}1_{, H. Cinli}1_{, M. Durmuş}1_{, M. Ünal, H. R. Kutlu}1

1 _{Agricultural Faculty, Department of Animal Science, Çukurova University, Adana, Turkey,}

Introduction

Spirulina platensis is an interesting source of important protein for both human and animal consumption, which is cyanobacteria, is a crucial functional food additive. Spirulina platensis which is a photosynthetic micro algae, contains high crude protein content (62-70%) as well as precious metabolites (Fox, 1996). Recent studies have propound that dry chicken manure can be used as low-cost nitrogen source in cultivation of Spirulina platensis (Ungsethaphand at al., 2007).

Dry chicken manure(DCM) contains most of elements particularly nitrogen (N) and phosphorus (P) which microalg need for growth and flourish. In Turkey, about 7 million tons of poultry manure creates environmental problems per year(Eleroğlu at al., 2013). The aim of study, cell density was counted and dry biomass were analyzed (DCM medium) was to compare the microbiological quality of Spirulina platensis cultured in standart medium and DCM medium.

Materials and methods

Pure Spirulina platensis culture was supplied from The Culture Collection of Algae and Protozoa (CCAP) / U.K. DCM was provided from Çukurova University Faculty of Agriculture, Research and Application Farm. DCM (200g/L), sodium bicarbonate (40mg/L) and sodium metabisulphite (5 mg/L) were added to DCM culture medium (Ungsethaphand at al., 2009; Ungsethaphand at al., 2007; Venkataraman at al 1969; Piorreck at al., 1984). Culture volumes were extended from 250 ml to 20 L. Cultures were illuminated with daylight fluorescents and aerated with air pump. The biomass was harvested by using 42 µm mesh filter at 22nd day of the cultivation when culture reached maximum density. Then algae was rinsed with tap water, dried in drying oven at 600C - 6 hours and floured. Dried Spirulina platensis was analyzed for nutrient contents (dry matter, crude oil, crude ash, crude protein) according to Weende method.

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490 The dried samples were microbiologically tested for the presence of bacteria such as, Salmonella spp., E.coli, Campliobacter spp., Coliform, Enterococcus spp., Lactic acid bacteria spp. Total count of aerobic mesophilic bacteria was also determined. Bacteriological test were by means of standard methods of isolation of individual species of bacteria as reported by ISO requirements.

Results

Growing parameters of Spirulina platensis cultivated in DCM medium (20 days); 421.8 mg/L cell, 3.40 mg/g chlorophyll-a density.

Nutrient composition of Spirulina platensis cultivated in DCM medium; 93.90 dry matter, 8.35 ash, 1.50 oil, 61.77 protein, 2.61 cellulose.

In the result as Salmonella, Campliobacter, E.coli, Coliform bacteria were not found in the both of control and treatment samples (DCM meculture medium). Enterecoccus spp. were found 3.91 log 10 cfu/gr for control, and 2.01 log10 CFU/gr for treatment groups respectively. Total count of aerobic mesophilic bacteria were determined 5.53 log10 CFU/gr for control and 2.98 log10 CFU/gr for treatment group respectively.

Conclusion

Depending on the growing conditions, Spirulina platensis contains crude protein, essential fatty acids, carbohydrates, vitamins, minerals, carotenes, phycocyanins, chlorophyll a (Vonshak, 2002). Spirulina, which has been important as ecologically and economically cell protein, is cultivated in poultry manure enriched medium, one of the environmental pollutants, affecting environmental recovery positively. It is important that the grown biomass does not carry microbial contamination of poultry that can cause disease for humans.

Acknowledgements

We would like to express our appreciation to the Çukurova University Scientific Research Project Commission, which supported this study (CUBAP-09/2017-9767).

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References

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