Effect of site of insemination on bovine fertility

v...ı.HlI.lk·r~.(I'N7). IJ.ı;-ıı-n

EFFECT OF SITE OF INSEMINATION ON BAVINE FERTILlTY

Mehmet

B. Ataman

1

Fikret Karaca

2 ıbrahimTaşal2

Abdullah

Kaya1

Me

li

h

Aksoy

1

Tevfik Tekeli

1

İn

ek

v

e

D

üv

elerde S

per

m

a

Ihr

akı l m a

Yeri

nin

Ferti

lite

Ü

zcr

in

c Etk

isi

Özet: Buçalışmada. sunitohumlamayapılaninek vedüvelerdespermabırakııma yerinin gebe likoranları üzerine et -kıs i araştı r ıldı. Çalışmada 82 ba ş inek ve 18 baş düve olmak üzere toplam 100 baş hayvan cervical,corpus uten. Graaf follikülü nünlokatizasvon unagöreipsilate ralvekontralateral cornu tohumlama bölgesi olmaküzere4eşitgruba

ayrı laraktohumland ı l ar. Gebeli koranları gruplardasırası yla%88,%72,'%84 ve%72olaraktespit edildi. Sonuçolarak. sperm anıncorpus

ut

e

n

veyaıpsi lat eralcom uya depoedilmesiningebelikora n ı nı arttırdığı.cervicalyollato h u ml amanın herhangi biravantaj ı olmadığı kanı sınavarıld ı.

Anahtarkelimeler:

lne

k.

sperına,bırakıımayeri.gebehkoranı

Summa ry: Effectofsiteofsemen deposition onpregnancyrates otartil iciallyinseminatedcatnewere investigated .A tota l ol100 animals, 82 cows and 18heıt e rs, were dividedintolourgroupsand inseminat eclby depositing semeninto corpusorcervixof theuterus oripsi-orccnuaıat e raı hemtotheGraffiantcücre .Pregnancy rateswere88.72.84and 72%. respectively.Itwas concluded that depesrtionol the semeninto ccrpcs uter!oripsitateral hem totheovutaıory lolliele increase pregn aney rateswithAland cervix uteri does not appear tobe advantageoustarget lorcente. Keywords:Bovine.semen deposition.pregnancy rate

Inlroduction

Since artificial insemination (AI) had been

employed on commercia l bovine hetds many te

-portshavebeen teleasedsuggestingdifferenl sites

of genilaltraetlot Ihe semen deposition.In 1950's intrace rvcal deposilion of semen was being r e-gatded as moreadvantageous eompared to intra

-uterine deposition method (Steward and Mel

-rose.1952). Aher a few years Olds ei al. (1953)

and Maepherson (1968) recommended uıerine

body for the depesinon of semen. Mora recently,

Mrlehell et al. (1985) reported that approximately

60%

of spermatozoa deposited inlo the uterine

body were lost by mueous diseharge from the g

e-nitaltract.Sengeret

el.

(1988) expressedlhal ca

n-ception rates were improved when

co

mua l

in -semination was employed and. thal comuel

inseminalion provides an altemalive lo ın­ saminalion in uterine body. Williams et aL. (1987)

GchşTarihi:12.0:U 99 7

ı.t'"açullyuf VetcrinaryMed icine.Umversrty ofSelçuk. KONYA.

2.Fa.:ulty of VctcrinaryMeda-ine.UniversityofYU/.UncÜYı L.VAN.

suggested thal cornual insemination does not t

e-sult in uterin e tra uma or bactertal canla minalion

and endometri um is capable of tegeneration atte r Irau ma during esrrus. Momonl el al. (1989)conc

-luded that comual inseminatio n has no advantage

overinseminaıionin theuterine

body

for ineteasin g

the.pregnarıcy

t

ates

.

The objective of the present study was lo d

e-te rmine the effect of site of semen depasilion on

pregnarıcy rates of call1e.

Mat e t ial s an d Method s

A lolal of

1

00

cattle, 82 cows and 18

b

eit

e

rs.

aged between 1.5-6 years old were selected from

theanimals whieh werebrou ght lo lhe Artificial in -sernineton Clinie for Al service. Those anunals were selected based on the eriterionslisted below.

a) no dvstocbia and reta ined fetal membranes in

va-Table1.Pregnancyrat eswıt hregards todıtlerenısrtesof Mmen deposition in cattle.

Dılle renısuperscriptsincolumndıt1ersıgnılicanlty (p<0.05)

Contra rily. Mo montet at. (1989) sugges l edthaı

pregnancy rat es for ipsilate ral and conrratatera! i n-seminations wer eequel.

Inconc lusion.deposilian of Ihe semen into co r-pus oripsi late ral hamofthe uterus ineteas ed pr eq-nancy tates with AIand cervix uıeri does ret

ap-pearlobeadvantageou starget forcanla.

References

Gallagher,G.R.andSenger,P.L. (1987):ccrceraretene ol spermaıo zoa in the ecvoe vagına lollowıng semınal

deposilionin the uterinehoms or utenne body J.Anim Sci.65(Suppl. 1),400(Abstr.).

Macpherson,J.W. (1968):Semen placemenl elfactson lerı il ityin bovines.J.DairySeL.51,807-808.

McKenna, T., Lenz. RW., Fenton. S,E. and Ax. R.L. (1990):Non-returnratesol daııy eettıe 10lloWlng U1enne body and cemuar insemination.J. Dairy Sci. 73, 1

n9

-1783.

Mıtchel l,J.R.. Senger, PL and Rosenberger.J.L. (1985): Oislribut ıo nand retenlion ofspermatozoawith acrosomal and nuelear abncrmaünes in the cow genllal tract. J. Anım. Sci.61,956-967.

Momont, H.W., Sequm. B.E, Singh,G. andStasıu kynas, E. (1989):Ooesinırauterine siteol loserrmancn incatlle reatty rnatter? Theriogenology 32.I.19-26,

aıds, 0.0 ., Sheath, D.M.. Carpenter, M.C. and Lucas,

HL (1953) : Interrelationships between site ol de-pcaiticn. dosageand nurroe r of spermatoıoa in diluted semen andferıility of dairy cows inseminaled arlılici alty. J.Daıry Sei.36,1031·1035.

Senger, PL , Becker, W.C., Davıdge, S.T., Hillers.J.K.

and geevee. J.J. (1988) : Inlluence of cemuel in-seminatıon on ccncecucn ın daıry canle.J. Anım. Ses. 66.3010-3016.

Senger,P.L.Becker. W.C.and Hillers,J-K.(1987):Cor

-nual !OMlTınalion increases concepı:ion rates in daıry canle.

J

.

Anim Sci.(Suppl.1),400 (Abstr.).

Steward, OL and Melrose, O.R. (1952) : The com-parativeol theintra-e eıvicaland intra-uterinemelhodsol in se minaııo nin the dairycow,Vel.Rec.64.605-607.

WiMiams.BL, Senger,PL and Oberg,JL (1987):Inf·

luenceot cornual lTlsemin atiorı on endometnal damage andmicrobial llora-inthebovıneuterus.J.Anım. Scı. 65.

212·216.

ATAMAN.TAŞALAKSOY.KAR ACA.KAYA .TEKELI

gina!examinatio nc) compleled50to90dayspost -partum d) hav ing palpable ovulatory loIliele al the time of

A

I

and that uterus retu rned its pr e-gesıational size and situalion e}

no

A

i

or mating etıeepreviouscalving.

Selected anima ls were randomly divided inlo fourgroups.

2

5

animalspergroup.to inse rrsnate

by

depositing

se

rren

tothe followingfour Iocaiıations_ 1) Uıerine body insemination: depositicın

of

semen into corpus Uıeri 2)

osu

a teeet

com ual insemi nation; deposition ol semen into com u uteri whieh is

e

d

-jacenı to ovulatoıyfollid e containing oııary 3) co

nt-ralareratcornual inse mina tion;depo sition of seme n into com u uten which is contrele teral LOovulatoıy tonere containingovary 4)cervicetinsemination; d e-position ol semen into rrsd-cervix uteri.

ALL

in

-serrmetooswereperformedby two inseminal ors by frozen-thawed seme n in

0

.25

ml

ol

straws lrom

peo-ven bulls.Pregna ntanimals were detected byrectal

palpalion60-90

da

ys

aherinseminations.

Da

la

were

anaIysed

by

Chi-sq<ı<e

anaises

lo

corrp:lf6Pfeg'lMlCY rates inpercentage between

go-..,.

Resultsand Discussion

Pregnancy rates with the regards lo semen deposilion sitas are slbmitted in table 1.The hig-hest pregnancy rete was obtai ned by the d e-position of the semen mtc uterin e body.How ever, no signilicant diffe renc e in pregnancy Tates was deıectedbetween cow s inseminated into ipsüatera l horn and uterine body. This data is in agreement with thelindings ol Mckenna et aL. (1990) who re -ported thal non-retum rates were simila r in cows

insemınated intoUıerinebody andcom u uteri.

Gal-Iagher and Senger (1987) pointcd out that site of sem ina l depositio n, ham or body of the uter us.

doesnol inlluence the back upol spe rmatozoa into the vagi na, Pregnancy rates achieved

by

cont-ralaıeral comual and cerv ical deposıtıon in this

study are significanllyIow erco~red lothose

ob-laıned by ipsilate ral com ual and Uıerine body in-seminations.Thisfind ing is inconsona nc ewith the reportsol Seng eretal. (1987) whosuggested that com ua l inseminal ian increase s conception ral es over uterin ebody insem inat ionsindaiıycanle.

Site ofsemet1 InsenYIated depositıon anımals Uterınebody 25

lps~ale ralhom 25 Ccnnatate ra!hom 25

Ceıvixuteri 25

4

2

Pregnant Pregnancyrate anlmalS (~,.) 22 88 .0-21 84 .0-18 72.(/> 18 72.(/>