Screening of anti-oxidative effects in Camellia sinensis L. leaves treated with boric acid

(1)

Işıl İsmailoğlu

a

_{, Zeynep Mine Coşkun}

b

_{, Melike Ersöz}

b

_{, Murat Ali Turan}

a_{Department of Biology, Faculty of Arts and Sciences, Marmara University, Istanbul, Turkey}

b_{Department of Molecular Biology and Genetics, Faculty of Arts and Sciences, Istanbul Bilim University, Istanbul, Turkey} c_{Department of Soil Science and Plant Nutrition, Faculty of Agriculture, Uludag University, Bursa, Turkey}

Camellia sinensis L. (tea) is one of the most widely consumed drink in the World. The anti-oxidant

role of boric acid has been reported. The present study was aimed to evaluate the alteration of anti-oxidative effects of C. sinensis L. leaves extract treated with boric acid.

INTRODUCTION

MATERIAL AND METHOD

C. sinensis was grown up in Rize, Turkey. The

land was divided into four group. Each group was occured five areas (10 m2). The first group

is control. Boric acid in concentration range of 100, 300, 500 mg/m2 in sodium tetraborate

buffer were applied as a single dose on the second, third and fourth groups, respectively in March 2013. C. sinensis leaves were collected on two different periods (May and July 2013). The levels of malondialdehyde (MDA) and reduced glutathione (GSH), the activities of superoxide dismutase (SOD) and catalase (CAT) were measured in C. sinensis leave samples.

RESULTS

MDA level in tea leaves showed a significant alteration among four groups at first period. At second period, it was seen that MDA level increased at 100 mg/m2 concentration of boric

acid although MDA level reduced at 300, 500

mg/m2 concentration of boric acid. There was a

significant change in GSH levels among all groups at first period. A difference did not

determined in SOD levels among 100, 300, 500

mg/m2 concentration of boric acid at first

period. However, CAT levels elevated at 500 mg/m2 concentration of boric acid at first period

(Table 1). Table 1: Biochemical parameters in all groups

First Period

Second Period

Control 100 300 500 P_ANOVA Control 100 300 500 P_ANOVA

MDA* (nmol/mg) 1.72 ± 0.31 0.87±0.06 a _{0.69 ± 0.10}a _{0.76 ± 0.09}a _{P˂0.05 1.23 ± 0.08 1.55 ± 0.14} _{1.09 ± 0.10}c _{1.38 ± 0.12} _NS GSH* (nmol/mg) 66.19 ± 11.64 27.02 ± 9.20b 18.73 ± 2.79a 14.93 ± 1.96a P˂0.05 57.43 ± 8.04 48.78 ± 3.71 38.26 ± 2.58b,c 51.45 ± 6.60 NS SOD* (U/mg) 3.51 ± 0.93 1.60 ± 0.21b 1.31 ± 0.24b 1.41 ± 0.15b NS 1.41 ± 0.19 1.99 ± 0.35 1.50 ± 0.13 1.78 ± 0.30 NS CAT* (U/mg) 4.21 ± 1.08 4.40 ± 0.55 4.65 ± 0.70 6.12 ± 0.19c NS 5.62 ± 1.51 3.32 ± 0.28 3.96 ± 1.11 4.84 ± 0.44d NS

*Mean ± Standart Error of Mean (SEM), NS: Non-significant

aP<0.01 versus control; bP<0.05 versus control; cP<0.05 versus 100 mg/m2; dP<0.01 versus 100

mg/m2

CONCLUSION

In conclusion, it is suggested that treated boric acid may be elevated antioxidant status of C.

sinensis at second collection period.

Screening of anti-oxidative

effects in

Camellia sinensis

L. leaves treated with boric acid

Screening of anti-oxidative

effects in

Camellia sinensis

L. leaves treated with boric acid

ACKNOWLEDGEMENT: We thank to Research Foundation of Marmara University (Number FEN-DKR-250405-0114) for financial support.